Journal: Indian Journal of Pharmaceutical Sciences

Article Title: Mechanism(s) Involved in Carbon Monoxide-releasing Molecule-2-mediated Cardioprotection During Ischaemia-reperfusion Injury in Isolated Rat Heart

doi: 10.4103/0250-474X.107047

Figure Lengend Snippet: Experimental design for CORM-2-mediated cardioprotection and role of various kinases. The isolated, Langendorff-perfused rat hearts were stabilised for 10 min, perfused for 15 min with K–H buffer. Hearts were treated with Vehicle or iCORM-2 (inactive form of CORM) or CORM-2 (50 μM) (a, b and c). The treatment with various inhibitors like SCIO-469 (1 μM) or SB-203580 (10 μM) or chelerythrine (10 μM) or wortmannin (100 nM) was initiated 5 min before CORM-2 administration and continued during CORM-2 treatment followed by 30 min of global ischaemia and 120 min of reperfusion (d, e and f). Some hearts (g, h and i) pretreated with CORM-2 and 100 nM wortmannin, the treatment with wortmannin was initiated 5 min before CORM-2 administration and continued during CORM-2 treatment and throughout reperfusion.

Article Snippet: Isolated heart was cannulated via aorta and perfused in the Langendorff's mode at constant perfusion pressure 70 mmHg using Radnoti Langendorff constant pressure non-recirculating system (Radnoti glass technology Inc., CA, USA).

Techniques: Isolation

Journal: Frontiers in Cardiovascular Medicine

Article Title: Simultaneous assessment of mechanical and electrical function in Langendorff-perfused ex-vivo mouse hearts

doi: 10.3389/fcvm.2023.1293032

Figure Lengend Snippet: List of equipment.

Article Snippet: Langendorff reservoir with glass tube gas disperser , Reservoir to collect and oxygenate buffer from the non-recirculating line , Radnoti LTD, Dublin, Ireland.

Techniques: Control, Software, Membrane

Journal: Frontiers in Cardiovascular Medicine

Article Title: Simultaneous assessment of mechanical and electrical function in Langendorff-perfused ex-vivo mouse hearts

doi: 10.3389/fcvm.2023.1293032

Figure Lengend Snippet: The Langendorff system. ( A ) General diagram of the Langendorff system and its different components. ( B ) Photo of a cannulated heart mounted on the Langendorff system and perfused via the aorta. The intracardiac octapolar catheter is inserted in the right atrium and right ventricle, while the balloon is inserted in the left ventricle after removal of the left atrium. 1 = pacing electrodes, 2 = intracardiac octapolar catheter, 3 = aortic cannula, 4 = intraventricular balloon system coupled to pressure measurement, 5 = silver wires, Ao, aorta, RV, right ventricle, LV, left ventricle. ( C ) Ischemia-reperfusion protocol during which the hearts are perfused aerobically for 20 min (pre-ischemia) before the 25 min global ischemia phase, during which perfusion is stopped, and the hearts are placed in an energy-substrate-free immersion bath. Hearts are then reperfused aerobically for 60 min. Red stars indicate the time points at which the ventricular effective refractory period was assessed by electrical pacing. Figures 1A–C were created with BioRender.com.

Article Snippet: Langendorff reservoir with glass tube gas disperser , Reservoir to collect and oxygenate buffer from the non-recirculating line , Radnoti LTD, Dublin, Ireland.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Simultaneous assessment of mechanical and electrical function in Langendorff-perfused ex-vivo mouse hearts

doi: 10.3389/fcvm.2023.1293032

Figure Lengend Snippet: Combined measurements of electrical and mechanical function on ex-vivo perfused mouse hearts. ( A ) Example trace recordings obtained from the intracardiac octapolar catheter (channel 1–6) and ECG recording with associated average heart rate, PR interval, QT interval, intra-atrial and intra-ventricular conduction times, and ventricular effective refractory period (VERP) at baseline from the ex-vivo perfused hearts ( N = 15). Data are expressed as mean ± standard deviation (SD). ( B ) Example trace recording of the left ventricular pressure and associated average developed pressure (DP), left ventricular (LV) work and maximal velocity of contraction (dP/dt max) and relaxation (dP/dt min) at baseline from the ex-vivo perfused hearts ( N = 15). Data are expressed as mean ± standard deviation (SD). ( C ) Schematic illustration of a Langendorff-perfused heart with the intracardiac octapolar catheter and corresponding electrodes 1–8 (Ch = channel) inserted in the right atrium and ventricle (1), silver wires to record the electrocardiogram (2), pacing electrodes to assess the VERP (3), and the balloon (4) inserted in the left ventricle coupled to pressure recording (5) to measure left ventricular pressure. Figure 2C was created with BioRender.com.

Article Snippet: Langendorff reservoir with glass tube gas disperser , Reservoir to collect and oxygenate buffer from the non-recirculating line , Radnoti LTD, Dublin, Ireland.

Techniques: Ex Vivo, Standard Deviation